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all dot regulated employers must perform dot drug testing using the approved dot five panel drug test with testing performed at a samsha certified laboratory as a dot-regulated employer youamp;#39;re required to drug test all d.o.t safety sensitive covered employees the most common examples are truck drivers bus drivers pilots and railroad employees you must follow the dot regulations for drug testing specifically 49 cfr part 40. this regulation describes the required procedures for conducting workplace drug and alcohol testing for the federally regulated transportation industry so what is the dot 5 panel test a common question we hear is what is the dot drug test for many years it was simply a five panel drug test and although there have been changes over the years it is still called a five panel drug test the standard five panel dot test includes the following drugs marijuana metabolites cocaine metabolites amphetamines opioids and phencyclidine for the dot five panel drug test we c