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hey guys this is Trini and thank you for watching these tutorials on my youtube channel Python for microscopist in todays tutorial Im going to talk about shading correction and Im sure most of you can relate to this and you may also think of this as non uniform illumination reduction for example if you are shining light from one side and you are imaging something and one of the you know one side of your image is brighter than the other how do you correct for this because this can affect your segmentation so for this Im going to show you two ways the first way is using contrast limiting adaptive histogram equalization see lhe in short and I talked about this in the past but lets just apply that and see if its doing a good job if not what is the other plan as you can tell from the screen there are actual plan is to use rolling ball background subtraction because it does an amazing job but first lets jump into our spider IDE okay so the image Im going to work with is this image t
