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in this video you will learn how to perform gram staining this technique utilizes a four step standing procedure with two different dyes and is one of the first tests used when trying to identify unknown species of bacteria using a wax pencil label a microscope slide with your initials then draw a circle about one centimeter in diameter on the slide using a metal inoculation loop sterilize it by flaming using aseptic technique at a drop of sterile water so that a film appears across the loop transfer the water into the circle drawn by the wax pencil flame the metal loop again to sterilize after allowing it to cool use the metal lube to transfer a colony of bacteria growing on an auger plate make sure to lightly touch the bacterial colony to avoid transferring too many bacteria when finished flame the loop to sterilize you let the liquid completely evaporate leaving behind a dry spot on the slide this will take at least 10 minutes once the liquid has dried completely heat fix the bacte