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sample preparation is one of the most critical part of the sex the goal of the sample preparation is to extract small molecule metabolome from a given biological sample while eliminating large molecule background coming from the proteins and also from the salt and other ions which can interfere during the mass spectrometry analysis the metabolome which is collection of all small molecule metabolites in a given biological system is very dynamic meaning it can change within minutes or within seconds this means if samples are not collected processed and stored appropriately the data obtained may not be a true representative of the samples at the time of the collection many factors such as type of sample collection method sample extraction protocol as storage need to be considered during experimental design even before you begin sample collection in addition to this collection and processing of sample may require specialized training and equipment some of whi