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[Music] hi my name is dr. Rob G and today Ill be demonstrating how to take blood cultures from a butterfly collection set its important to always remember to take blood cultures from a peripheral vein wherever possible even if theres other forms of access already in place such as an existing peripheral cannula or a central or femoral line its important that the site that we take the blood cultures from is kept clean healthy and is not broken and thats an aseptic non-touch technique is used the first step is to wash our hands its important that we use the seven-step hand washing technique and that we use this technique every time we decontaminate our hands in the hospital setting next we can prepare our kit you will need a sharp spin a trolley a tray in your blood culture kit this consists of two bottles a butterfly needle a blood collection adaptor cup a 2% chlorhexidine alcohol applicator and a 2% chlorhexidine wipe its important to check the expiry date on your blood culture b