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Testing for pregnancy has significantly evolved over the last 50 years, but certain situations, such as fertility treatments, can complicate result interpretation. During in vitro fertilization (IVF) with a fresh embryo transfer, understanding the medications used is crucial for accurate results. The final medications before IVF egg retrieval are known as trigger injections, with three main options: 1) hCG (human chorionic gonadotropins) alone, with brands like Pregnyl or Ovidrel; 2) GnRH agonists, including Lupron or Suprefact; 3) a combination of both. Knowing these details is essential for proper interpretation of pregnancy tests.