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[Music] hi guys welcome back to my channel i am so happy to see you all here so in this video we will be talking about finding differentially expressed features and cluster identification in single cell rna seq data we will be using that data to identify cluster markers using the functions that serrat package provides so i will be talking about each of those functions and also will be demonstrating how to use those functions and in what context or scenarios using those functions make the most sense so getting straight to the point lets say you have the single cell rna seq data you have processed it and ultimately clustered the data so your cells have grouped together into clusters and from then on you can ask various questions so lets say the first question you ask is i have two clusters and i want to compare between the two clusters and want to identify genes differentially expressed in one cluster versus the other so in that case find markers function makes the most sense because i