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welcome to the applied biosystems tech talk video series where we break down real-time pcr to make it easier for you housekeeping genes normalizers reference genes internal controls no matter what you call them weamp;#39;re talking about endogenous controls if youamp;#39;re using relative quantification to analyze gene expression with real-time pcr then you are likely already using endogenous controls but are you using the best control for your experiment letamp;#39;s talk about it when you use the comparative ct method to perform relative quantification a difference in expression level is measured as the difference between the delta cts of the test gene and the endogenous control for this calculation to be valid the endogenous control gene must be stably expressed for all studied samples treatments or time points to provide a reliable basis for comparison to find the best endogenous control we need to identify a gene with expression that does not vary under your specific ex
