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to see how a cell repairs a double strand break in DNA by non-homologous enjoining letamp;#39;s imagine that we have a parental DNA duplex containing the sequence a b CDE e we then imagine that the duplex is broken by a double strand break in the C region degradation of regions from each side of the break which are generally less than 10 base pairs long may occur this process is sometimes called resection Q protein binds around the broken ends leaving the actual DNA ends exposed coup also recruits the DNA PK catalytic subunit also called DNA pkcs DNA pkcs recruits the nucleus artamis and phosphates it artmus trims any single stranded tails that are present at the break lias 4 acting in complex with xrcc4 and XLF cunos legates the broken ends together as a result the duplex is rejoined but note that any region that was removed by a nuclease will be missing in the repair duplex in this case joining does not restore the DNA to its pre-break AB CDE sequence because the C region was remove
