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this is a video of a 3d animation on the mismatch repair pathway in e.coli in the cytoplasm is the Eco lies DNA the molecule that contains all of its genetic information DNA is made up of nucleotides each of which contains a phosphate sugar backbone and an organic base the two strands of DNA are held together by hydrogen bonding between complementary base pairs adenine pairs with thymine and cytosine pairs with guanine DNA mismatches occur during DNA replication for simplicity this animation will show DNA as a flat molecule before replication can begin a helicase needs to unwind the DNA molecule in e.coli DNA polymerase 3 catalyzes the incorporation of new complementary bases to the new DNA strand this is only the Alpha and Beta unit of the DNA polymerase 3 enzyme however it is possible for DNA polymerase 3 to incorporate a non complementary base to the new strand this is known as a mismatch a mismatch could result in a faulty protein being coded for so it needs to be repaired the firs