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the simple stain is a straightforward method for differentiating microbial cells from a background it involves exposing a smear to a single Dy and then washing it off a smear is prepared as described in the smear preparation movie make sure the slide is allowed to dry completely and then heat fixed before staining when preparing slides patience is a virtue after a smear is prepared the slide is placed on a staining tray stain is added in a dropwise fashion until the smear is completely covered many stains can be used for simple stains it only changes the colors of the cells here we are using safranin the stain remains in the slide for a few minutes depending upon the protocol note that it is not important to completely cover the slide just completely completely cover the smear after the appropriate time the slide is tilted and much of the stain runs off the stain is then rinsed off with water and blotted dry it is useful to tap the edge of the slide on the blotting paper to remove move
