Revise stain in MCW

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How to revise stain in MCW

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Welcome to our new video that is mcqs on microscope stain and staining techniques and this is the third part of this series of videos on microscope stain and staining techniques okay so the first question is which instrument is used to determine the turbidity and indirectly the bacterial count of a given sample and options are Zeta Sizer Zeta scissor or spectrophotometer or centrifuge or all of the above and the right answer is option b the respect of photometer so specto phytometer instrument is used to determine the turbidity and indirectly the bacterial count of a given sample so the next question vector so next question here measuring the metabolic activity of a bacterial sample provides direct count of the bacteria indirect count of the bacteria both A and B are none of the above and right answer is option b that is indirect count of the bacteria so measuring the metabolic activity of a bacterial sample provides indirect count of the bacteria okay so next question they can get sta

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What is the mordant in capsule staining?

What is the capsule mordant? The capsule mordant contains a stain called acid fuchsin. The acid pH of the mordant changes Congo red to a blue color. What does the acid fuchsin in the capsule mordant do? The acid fuchsin stains the bacterial cells a pink color.

What does the counterstain dye in a capsular stain?

The counterstaining is provided by Congo red. In the procedure for Manevals capsule staining method, the bacterial cells are first introduced in to a drop of Congo red solution on a slide. Congo red is a pH indicator that is blue at or below pH 3.0 and red above pH 5.2.

What is the purpose of the counterstain in staining?

In general, counterstains are designed to provide a contrast of nucleus with cytoplasmic immunostaining and surrounding tissue.

Which reagents are used for staining MCQ?

Explanation: Gram staining is a type of differential staining. In this process the fixed bacterial smear is subjected to the following staining reagents in the order listed: crystal violet, iodine solution, alcohol (decolorizing agent), and safranin.

What is the counterstain in capsule staining?

The primary stain applied is crystal violet, which stains both the bacterial cell and the surrounding capsule. A 20% copper sulfate solution is then applied, which serves a dual function as both decolorizer and counterstain. It removes and replaces the crystal violet in the capsule only.

What dye is used in a capsule stain?

In Anthonys capsule stain, crystal violet is used as the primary stain, interacting with the protein material in the culture broth or added during the staining, and copper sulfate serves as the mordant.

How would that change the outcome of the stain if you were to forget to add crystal violet?

Without the crystal violet staining, it would be impossible to differentiate between Gram-positive and Gram-negative bacteria, as the secondary counterstain, usually safranin, would stain all cells pink instead of just the decolorized Gram-negative cells.

What is the initial stain used in the acid fast stain multiple choice question?

Acid-Fast Stains Both use carbolfuchsin as the primary stain. The waxy, acid-fast cells retain the carbolfuchsin even after a decolorizing agent (an acid-alcohol solution) is applied. A secondary counterstain, methylene blue, is then applied, which renders nonacid-fast cells blue.

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