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rapid game sustaining of air dried spread for adequacy assessment in our department the following protocol is used this menu to be altered ing to local stains utilized the air dry sprayed is placed in solution a methanol for two minutes remove the slide and place it in solution B eosin 47 seconds then place in solution see methylene blue for 15 seconds after this place in solution d buffered water at ph 6.8 for 45 seconds finally place the air dried sprayed into dear ionized water for 45 seconds remove the slide from the deionized water and place onto filter paper gently Pat the slide try using the filter paper screen the slide on the microscope for adequacy assessment if the stain slide is adequate it is not necessary to stay in the other air-dried spread therefore to aspirates from one side yielded one stained air drives bread and one fixed spread from the first aspirate with one unstained a dried spread and one fixed spread from the second desperate
