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tapi is a very well known nuclear dye for use in many applications as a relatively impermeable dye Dappy is the optimal choice sustain the nuclei of fixed cells when Dappy is in close proximity to double-stranded DNA it binds to the minor groove of adenine and thymine rich regions upon excitation in the ultraviolet range of the light spectra Dappy releases a bright blue fluorescence that can be detected by a blue filter pure blue Dappy can be used with any cell imaging systems containing a UV excitation source and a blue filter such as bio-radamp;#39;s news OE cell imaging system pure blue Dappy is packaged in easy-to-use vials that can be diluted to the typical working concentration of one microgram per mil in a single step these HeLa cells have been fixed with 3.7 percent formaldehyde at room temperature for 10 minutes and are now in PBS to stain your fixed cells first create a working solution take one vial of your pure blue Dappy dye and add 500 microliters of deionized water to g
