Revise stain in AFP

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Aug 6th, 2022
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How to revise stain in AFP

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hi everyone in this video we will have a short discussion on how to report staining results letamp;#39;s have a short review on our gram staining procedure the first step is application of your primary dye in this case crystal violet this is followed by your mordant which is iodine and then the most important step decolorization using alcohol and finally you counter stain with your safrini crystal violet as the name suggests is purple in color while safranin is pinkish to reddish in color here are the expected results for gram-positive bacteria they stain a purple color well for gram-negative bacteria they stain from pink to reddish next we have acid fast staining the procedure for this is different from your gram stain reaction first you apply your primary stain which is carbofuxine this stain is pink and or reddish in color then instead of adding iodine you use heat as your mordan followed by decolorization with your acid alcohol then finally you apply your counter stain which is me

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Generally, an AFP level between 0 ng/mL to 10 ng/ml or even up to 40 ng/mL can be normal for adults But normal levels vary, so talk with your healthcare provider about what your results mean for you. An extremely high level of AFP in your bloodgreater than 400 ng/mLcould be a sign of liver tumors.
The other cases of false-positive AFP elevation have been reported in the male counterpart of MOGCTs, TGCTs. The most common cause is liver injury secondary to alcoholism, drugs and hepatic virus infection, often manifesting as abnormal liver function tests [26].
Incorrect Dating: A pregnancy that is further along than believed at the time of the test would make the AFP appear elevated. Also, twin and triplet pregnancies create more AFP, increasing the blood levels and making the test results appear abnormal.
Alpha-fetoprotein tumor marker Alpha-fetoprotein (AFP) is the most widely used tumor marker for detecting liver cancer. However, other cancers and certain conditions, including pregnancy, hepatitis, and other types of cancer, may also increase AFP levels.
A slightly elevated (6‑19 ng/ml) AFP level may be a risk factor for HCC in certain cases. By contrast, AFP levels
AFP alone is inadequate for screening purposes because of the high rate of false positives in active hepatitis; it has only 40-64% sensitivity because many tumors do not produce AFP at all or do so only at a very advanced stage.
The study by Harada T, et al. [39] showed that approximately 40% of all cirrhotic patients will have AFP levels higher than 20 ng/ml. An increase in AFP levels (between 10 and 500 ng/ml, and sometimes up to 1000 ng/ml) can be seen in adult patients with hepatitis or cirrhosis with any etiology.
Alpha-fetoprotein (AFP) is determined as a tumor marker for hepatocellular carcinoma and yolk sac tumors. Recently, cases of gastrointestinal cancer with elevated serum AFP levels have been reported.

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