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stat quest stat quest stat quest hello and welcome to stat quest stat quest is brought to you by the friendly folks in the genetics department at the University of North Carolina at Chapel Hill today weamp;#39;re going to be talking about rpkm versus fpkm versus fpm in previous stat quests weamp;#39;ve talked about topics that are broadly applicable to a variety of fields these subjects however really only relate to high-throughput sequencing of RNA so if thatamp;#39;s what youamp;#39;re interested in quest on thereamp;#39;s a new RNA seek metric on the block we used to report rpkm or reads per kilobase million or fpkm fragments per kilobase million these normalized read counts for the sequencing depth thatamp;#39;s the million part because sequencing runs with more depth will have more reads mapping to each gene that in sequencing runs with less depth and we donamp;#39;t want that to bias our analysis the other thing that these metrics normalized for are the lengths of genes th
