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hi everyone jason kirk here from the optical imaging and vital microscopy core at the baylor college of medicine today weamp;#39;re going to discuss how to properly use immersion objectives on our inverted microscopes weamp;#39;re going to show you how to safely mount your specimens using an oil immersion objective and provide some tips and tricks to help you keep your samples and our microscope safe before we get into the practical use of these lenses letamp;#39;s start off by discussing a bit about why we need to use immersion optics in the first place when choosing an objective for our experiments one of the most important questions we have to ask ourselves is what resolution do we need to properly analyze our specimen for example if your goal is to analyze the distribution of tumor cells within the adult mouse brain your resolution requirements will be quite different from someone whose experiment requires measurements of dendritic spine morphology understanding the size of the