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(squeaking) (ding) - I wanted to check back with you regarding my project on testing for differences in TNF alpha levels after spinal cord injury between rats treated by a drug versus those that did not receive the treatment. What is the sample size required for your calculations? - Well for the sample size and power calculations, you will need a total sample size of at least 30. - Okay so I have 10 rats that I can use, and Ill extract three samples from each rat and then that will give me a sample size of 30? - 30 measurements from 10 rats will not meet your goal of sample size. You will need 30 independent rats. - Oh, okay. So why is that? - If you take three measurements from the same rat, then these three measurements are not independent. Those three measurements generated from the same rat are really technical replicates. By technical replicates we mean multiple measurements from the same sampling unit, in your case, rats. These technical replicates represent independent measure

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How many replicates are needed for statistical analysis?

Biological replicates are required if inference on the population is to be made, with three biological replicates being the minimum for any inferential analysis.

How do you determine sample size?

Five steps to finding your sample size Define population size or number of people. Designate your margin of error. Determine your confidence level. Predict expected variance. Finalize your sample size.

What is a good number of replicates?

At least six replicates per condition for all experiments. At least 12 replicates per condition for experiments where identifying the majority of all DE genes is important. For experiments with 12 replicates per condition; use DESeq.

How do you find the number of replications in an experiment?

Page 1 Formula for Calculating Number of Replicates. r = number of reps. CV = coefficient of variation. t = tabular t value for a specified level of significance and df for error. t = tabular t value for df for error and a probability of 2(1-P), where P is. Options for Obtaining the Desired Number of Replications.

What does replicates mean in biology?

Broadly speaking, biological replicates are biologically distinct samples (e.g. the same type of organism treated or grown in the same conditions), which show biological variation; technical replicates are repeated measurements of a sample, which show variation of the measuring equipment and protocols.

How many times should you replicate an experiment?

Three repeats of an experiment is generally considered the minimum. Why? There are two reasons, the first has to do with the fact that three repeats ensures a two-thirds (66%) probability that the averaged results are more accurate than a single experiment.

How many replications are needed in an experiment?

At least six replicates per condition for all experiments.

What does 3 replicates mean?

verb. To make three copies of. adjective. Having three copies; triple; threefold. Supplement.

What are the replicates in an experiment?

What is a replicate? Replicates are multiple experimental runs with the same factor settings (levels). Replicates are subject to the same sources of variability, independently of each other. You can replicate combinations of factor levels, groups of factor level combinations, or entire designs.

Is 2 technical replicates enough?

STATISTICAL DESIGN (2008), an experiment ideally should encompass at least three independent biological replicates of each treatment. For each biological replicate, it is common to run at least two technical replicates of each PCR reaction.

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