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please read important safety information before watching this video this training module covers the procedure for replacing the sample needle on a nexgen micro lc200 using the keypad terminal press f1 for menu press f1 again to access change syringe the injector then moves to a position that is convenient for accessing the needle lift up the bottom needle guide until it touches the upper needle guide unscrew the knurled retaining nut and remove the needle by pulling the needle out of the needle guide prepare the new needle and teflon seal part number 4460864 by fitting the teflon seal onto the top of the needle and inserting the needle into the knurled retaining nut lift up the bottom needle guide until it touches the upper needle guide and insert the needle through both guides tighten the knurled retaining nut until finger tight using the keypad terminal press the inner circular button to continue the injection unit will then home once replaced the needle penetration depth should be c