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[Music] there are a couple situations when youll need to change the sample line on your scitec aurora or northern light cytometer one is when the line has become kinked or badly bent as this will cause sample flow instability the other is when the line has become badly clogged which youll know when youre trying to acquire and your flow rates are consistently near zero and no amount of cleaning is helping to change the sample line all you need is a new one which you can order from our website at scitecbio.com using the part information shown here with your cytometer powered off go ahead and open up your system so you can open the sit access door remove the tube from the cytometer then grab this metal part on both its sides and gently glide it down so you can better access the components next disconnect the black plastic nut at the top that connects the sample line into the flow cell lefty loosey once disconnected go to the other end and disconnect the beige nut when loosening this on