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hi my name is um hassan razvi im a consultant hematopathologist at barts and im going to be talking about cytochemical stains in hematology so before we start im just going to cover what uh the next 40 to 45 minutes will deal with ill just describe what cytochemical stains are how we got to you know doing these stains in hematology what are the particulars of each stain type and then what are the uses in hematology so before we get into any details about cytochemical stains i just want to talk about how pathology practice and classification of disease has developed over the last couple of hundred years so initially if you look at papers like thomas hodgkins landmark paper which describes the lymphoma that he had you know put together from a cased series of patients it was based purely on macroscopic and autopsy studies then obviously we come to the era of the microscope and morphological descriptions of disease based on cytomorphology or histology in the 1960s 70s we start the de