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[Music] so welcome to this demonstration of the H and E stay so the first step is after taking those slides down to water we then shake off the water and then place each of the slides into the Copeland jar containing in this case Ehrlichs hematoxylin now thats a regressive formulation of hematoxylin which means that we will be initially over staining those slides and then through a differentiation step will then be able to bring the level of staining back to the desired level now we leave it in there for about 10 minutes and a good way to keep track of time as you can see here is to put some masking tape out on the bench and write down the time when thats due to come off so after 10 minutes we then rinse those slides now you can put the slides directly into the smaller water bath which quickly makes that that water bath fairly dirty so its actually easier less work if you rinse off the excess initially just with the with the hose there in the largest sink and then just transfer th
