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There are various methods to remove a coverslip from old silde. So in this video I will explain 3 most effective methods for day today laboratory usage. Method 1 The most common method is the use of xylene. To recover the slide place it in a coplin jar cantaining xylene and close the lid. I t can be dipped in xylene from few minutes to several days depend on the age of the slide. Gently remove the coverslip once it has been loosened by xylene. Method 2 If the slide is too old, rapid most easiest method is , Place it in the freezing compartment of the refrigerator. As the coverslip and the mountant contract at different rate, the coverslip may pop off from the slide. Method 3 Another important effective , method is to spray the coverslip with freeze spray. The coverslip can be removed as the coverslip contract quickly than that of the glass slide.