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[Music] this video shows how to correctly remove the sample holder from the minitem microscope using the sample exchange wizard when the mini tem is not in use the sample holder should be stored inside the system without the grid thus the first procedure is to remove the sample holder from the imaging tab click on the sample exchange icon then choose remove sample holder this will start the turbo molecular pump spinning up which makes a loud sound and may take a few minutes a window pops up on the screen to guide you through the process four monitoring tabs show the status of the beam stage pump and air lock first is a reminder to open the airlock valve if it is not already open verify the airlock valve is open the minitem logo light on the front of the machine should begin blinking if the beam is on it will be turned off as part of the change sample wizard the beam will take a few minutes to shut down and then the monitoring tab will turn green and read beam okay when the stage values