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[Music] this video shows how to correctly remove the sample holder from the mini Tim microscope using the sample exchange wizard when the mini Tim is not in use the sample holder should be stored inside in the system without the grid thus the first procedure is to remove the sample holder before you begin be sure the beam is off the icon should be black the sample holder is removed and inserted through an airlock it is necessary to monitor the vacuum inside this airlock with the airlock chamber attribute so before starting it is important to navigate there under imaging tab microscope panel from the imaging tab click on the sample exchange icon then choose eject sample holder a window pops up on the screen to guide you through the process first as a reminder to open the airlock valve if it is not already open verify the airlock valve is open and click next a progress bar then shows the status of the turbo molecular pump spinning up this makes a loud sound and may take a few minutes the