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this video was produced by researchers at the university of georgiaamp;#39;s warnell school of forestry and natural resources the purpose of this training video is to detail how to process our loblolly pine pt gen2 cdna microarray paying particular attention to the washing and handling of the array both before and after hybridization in general our protocols follow those developed at the institute for genomic research tiger and the vanderbilt microarray shared resource facility vmsr where our arrays are printed we have found that more stringent processing of ptgen2 is required to provide the greatest amount of consistency with respect to uniformity of signal quality and low backgrounds the first step is a pre-wash we do this because our spotting buffer contains a very high salt content and this pre-wash is necessary to remove that salt the slides are placed in a microscope slide carrier and plunge to 20 times in a 0.2 percent sds solution that has been preheated to 43 degrees after th