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now youamp;#39;ll learn about the limo package for differential expression using the limo package for differential expression has multiple clear advantages over writing your own bespoke code first testing thousands of genes individually would require writing a loop with boilerplate code like counting variables thus obscuring the intent of the analysis every limo function performs its action on every gene in the dataset making it easier for you to write the code and easier for others to understand your analysis but itamp;#39;s not only more convenient for you itamp;#39;s also better statistics the loop in the code above treats every gene as a completely independent analysis but because all the genes were measured in the same experiment limo uses a statistical technique known as empirical base to share information across the genes which is especially helpful for studies with small sample sizes now empirical Bayes methods would deserve its own separate course so I wonamp;#39;t elabora
