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welcome and thank you for taking the time to watch the following presentation on reverse transcription quantitative PCR my name is Gillian Brian and Im a global market development scientist for our gene editing and novel modalities team supporting our gene editing tools and the workflows in which these tools are used including molecular biology during this presentation I will briefly introduce quantitative PCR technology and sets you can take to improve your qpc or based experiment I will then give a brief overview of the fundamentals of reverse transcription qpc or some common issues that can occur while performing or TQ PCR and highlight how reagents like thermistor 40 can help enhance your or tepees your results please feel free to submit questions during the presentation and we will follow up with you by email afterwards theres kick things off here lets start with a brief review of some of the basics so real-time PCR also known as real-time quantitative or qPCR determines the ac