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in this video we are going to show you how to inoculate a sterile liquid culture medium with a bacterial colony for this technique you are going to need a test tube containing sterile nutrient broth your bacterial culture on a solid culture medium and a culture loop also known as an inoculating loop before working with it make sure the wire loop is straight by running it between your thumb and forefinger there are two ways in which we hold the inoculating loop like a pencil and like a paintbrush I will show you which grip to use at each stage so letamp;#39;s get started activate the blue flame on your Bunsen by turning the collar at the bottom of the burner and sterilize your loop by passing it through the hottest part of the Bunsen flame allow your loop to cool before proceeding pick up the part of the plate containing your bacterial culture you can leave the lid of the plate on the bench and holding your inoculating loop like a pencil collect a small sample of bacteria from
