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hi there my name is Danny buckin Iamp;#39;m the friendly sample preparation specialist here at phenomena amp;#39;kx specialize in with clinical and toxicology laboratories and today Iamp;#39;d like to give you a little helpful tip I know sometimes SPE can be a little bit frustrating and finicky but there might be a couple of things that we could do that could make your lives a lot easier and today this tip is slow is safe and what we mean by that is then when we load our sample into an SPG cartridge here we have a strata X C strong cation exchange cartridge we want to load the sample very very carefully and what we do is one milliliter per minute which translates to one drop per second so has a sample goes through you can count the drops as they come out one-mississippi two-mississippi and the reason we load slowly is so we give the analyte time to interact with the stationary phase and so that we maximize our recovery and similarly we do the exact same thing when we elute the sampl