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hey guys thank you for watching these tutorials on my youtube channel Python for microscopist in todayamp;#39;s tutorial Iamp;#39;m going to talk about sub pixel image registration in Python and if you ever worked on a flip sim especially you probably know what Iamp;#39;m talking about I mean you are collecting images slicing your sample collecting images slicing it and then now you want to register those images within even within a pixel right thatamp;#39;s where the sub pixel image registration actually comes comes into picture now even on light microscope if youamp;#39;re actually collecting images and you know putting the sample back and then collecting more images and putting the sample back collecting more images you know after doing something to the sample maybe slicing it or something then you may run into this need for sub pixel image registration so letamp;#39;s jump in and before actually jumping into the spider IDE Iamp;#39;d like to mention at least the paper on whi