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when using liquid media we have to take care to avoid the production of aerosols aerosols are fine droplets of liquid capable of floating in the air and are easily inhaled they can be produced by shaking or generally manipulating liquids as always you should work close to the Bunsen flame and avoid putting any lives down on the bench as they might become contaminated the broth container is first labeled with the information previously described and itamp;#39;s topless and a Coursera Lube issues to pick off a few single colonies from a pure culture grown on a solid medium the other hand picks at the bottle and itamp;#39;s ladies removed once opened the bottle neck is flamed this flaming draws any aerosols in the bottle into the Bunsen burner flame the bottle is slightly tilted and the inoculum on the leaf is rubbed onto the inside of the glass bottle so that when the bottle is upright the inoculum is submerged the bottle neck is then flamed once more and the ladies replaced finally th