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the tech man HP se scorecard panel is a qPCR based assay that measures expression of pluripotency and germ layer specific markers with this set of genes you can accurately evaluate your pluripotent stem cells this video will show you how to prepare and load your HP SC spore card plate to get started you can use human embryonic stem cells or human induced pluripotent stem cells IPS fees that are growing on feeders or in feeder free conditions you can also run this assay on embryo eight bodies the panel will accurately predict lineage bias from samples taken as early as day five the Q PCR reaction for the HP SD score card panel can be run either on a 96-well plate or a 384-well plate the 96-well plate can fit one sample and the 384-well plate can fit four samples to set up the qrt-pcr reactions simply add TaqMan gene expression master mix to youamp;#39;re prepared cDNA we recommend setting up your reverse transcription reactions of one sample per eight to PCR strength to make this step