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this tatch tip will discuss appending ITC data this includes the actual experimental technique as well as the data analysis associated with this process upon the addition of a full syringe full of tiran into TI Trend and the data has not gone completion meaning that the last few injections are not just a heat of dilution the Bret handle can be removed and the titration syringe containing the titrant can be refilled itamp;#39;s refilled loaded back into the buet handle quickly wiped and then Place back into the instrument now a second syringe full of the entire syringe of titran into titran is delivered at the end of this experiment the two sets of data are appended and then analyzed as one set of data in nanoanalyze appending two sets of ITC data can be accomplished by exporting the raw data appending externally and then importing the data back into nanoanalyze for analysis the first step is to open the necessary files this includes Li into macromolecule and then the second file the L