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in this technical video we will demonstrate how to use step mate to obtain PBMCs from whole blood in just 15 minutes start by smoothly pipetting 15 mils of density gradient medium into the set mate tube through the central hole in the insert bubbles may be present in a medium but this will not affect performance next dilute the blood sample with an equal volume of PBS containing 2% FBS keeping the separate tube in the upright position pipette the sample quickly down the side of the tube sup mate keeps the sample separated from the density gradient medium next Center huge the samples at 1200 times G for 10 minutes at room temperature with the break on Sep mate prevents the layers from mixing as the centrifuge slows down red blood cells and granulocytes pellet to the bottom of the tube during centrifugation while PBMCs remain above the insert some red blood cells may be present on the surface of the insert but these cells will not affect the purity of your PBM seeds harvest the P
