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qpcr quantitative pcr or real-time pcr is an effective technique to quantitatively determine the presence of a target sequence or gene in a sample up to date qpcr is the gold standard method to test patients for viral infections such as covid19 for that a swap is taken which may contain viral material the rna is extracted and reversed transcribed into dna the dna can then be analyzed with qpcr in a light cycler in conventional pcr a dna sequence of interest is amplified the presence or absence of that dna sequence is detected in an endpoint analysis after reaction completion during quantitative pcr or real-time pcr the accumulation of the amplification product is visualized with fluorescent reporters and the reaction progression is monitored after each cycle in qpcr the product amplification is therefore observed in a quantitative perspective with each pcr cycle the amount of pcr product doubles which can be detected as an increase in fluorescence this generates a classic qpcr curve a
