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thank you the overall goal of this technique is to extract bacterial periplasmic contents by cell fractionation for biophysical characterization including x-ray fluorescence X-ray crystallography and radio metal uptake this method can help us understand key biological relationships including structure function relationships of ABC Transport Systems and how they recognize their substrates specifically and transport them into the cell through the lipid bilayer the main advantage of this technique is to separately evaluate the cell contents and to extract protein from its native environment with minimal cross-contamination we first have the idea for this method while trying to remove yfe substrate realizing instead of using an artificial approach we could use the cell to produce apoprotein in addition to providing insight into substrate binding and transport across bacterial cell compartments methods like this radio Tracer assay apply to eukaryotic systems which also have
