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hello this is tim sandal and in todayamp;#39;s video weamp;#39;re going to be looking at performing gram staining as the beginning method to work out what types of microorganisms we may have recovered from the facility okay weamp;#39;re going to begin with having a look at the theory behind the gram stain and then weamp;#39;re going to have a look at a video made in the microbiology laboratory of victoire carrying out a gram state okay so with the um gram stain um it dates back to 1884. and there was a danish bacteriologist called christian graham who was examining lung tissue from patients who died from pneumonia and he discovered that certain strains of bacteria were preferentially taken up and retained by different bacterial cells and it was found that gram-positive bacteria have a thick mesh-like cell wall made up of a substance called peptidoglycan and this can make up about 50 to 90 percent of the cell wall and these organisms stain purple gram-negative bacter