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hi everyone right now weamp;#39;re going to go over a few techniques that youamp;#39;ll be commonly using in lab these techniques especially the gram stain and the heat fixation will be very important when you guys get to your unknowns so with that letamp;#39;s just jump right into it first as you can see weamp;#39;re going to go over heat fixation and what heat fixation does is it makes the bacteria stick to the slide better and the way that we would do that is we would first add the bacteria to the slide so either if itamp;#39;s um in a test tube weamp;#39;ll just take it right out of the broth and you know take anywhere from one to three loop fulls and let that dry typically the um if you know that your bacteria is gram-negative youamp;#39;ll use more loop fulls just because that gram-negative cells are much smaller than gram-positive cells all right after that weamp;#39;ll let them air dry and make sure that when you do this that theyamp;#39;re comp before you proceed to t