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gram stains are a relatively crude but quite useful technique for identifying and classifying microorganisms into one of two groups based upon their retention of crystal violet and iodine complex nevertheless the the nature of this technique requires a special attention to the use of control sections the mechanism underlying this differential staining of so-called negative and positive organisms becomes clearer when considering the generic structure of the the outer cell wall so in the case of a negative organism as demonstrated here in this cartoon the thin layer of peptico glycan sandwich in between the two plasma membranes holds relatively little content of the crystal violet iodine complex and so it can be differentiated more quickly on the other hand positive organisms as shown in this diagram have a very thick layer of pepticoglycan and so weamp;#39;ll retain far more of the crystal violet iodine complex when exposed to organic solvents gram-staining protocols are theref
