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the gram stain is named after its inventor hans christian graham as a danish botanist and teacher of pharmacology he devised this important method of differentiating bacteria in 1884 that is still in common usage today the gram stain is used to distinguish between gram-positive and gram-negative cells it is the most commonly used method to observe bacteria the gram stain provides two important features the gram reaction or color of the cell and the cell morphology the shape of the cell bacterial cells can be spherical rod or corkscrew in shape and nearly all clinically docHub bacteria can be differentiated into these three groups knowing which type is causing an infection is critical to patient care the gram stain differentiates cells based upon differences in the structure of the cell wall the cell wall is made up of peptidoglycan which consists of sugars and amino acids that form a layer around the plasma membrane of most bacteria bacteria that stain dark blue purple are
