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negative staining negative staining is a technique used in electron microscopy and light microscopy to visualize the structure of cells bacteria viruses and other biological samples it involves staining the background or the area surrounding the sample with a contrasting die leaving the sample unstained principle it is based on the fact that certain dyes such as India ink or nigrosin are negatively charged and are repelled by the negatively charged surface of the cell or bacterial membrane this means that the dye will be excluded from the sample and will instead stain the background allowing the sample to stand out in contrast procedure step one the sample is placed on a microscope slide and a small drop of the staining solution is added to the side of the sample Step 2 the staining solution is then gently drawn over the sample with a small piece of filter paper leaving a thin film of stain over the surrounding area step 3 the slide is then allowed to air dry and the sample is ready to
