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(English captions by Andrea Matsumoto, University of Michigan.) This program will explain how the gram stain procedure is able to distinguish between gram-positive and gram-negative bacteria by representing the staining events at the ultra-structural level. This is one animation, from a series of two, that specifically shows the staining of gram-negative bacteria with the critical structures of the bacterial surface represented schematically. The circle at the lower right tracks how the bacteria would appear in the microscope if they were examined during each step of the staining procedure. Prior to staining the bacteria would be transparent and invisible. After heat fixing the slide, it is first flooded with crystal violet for one minute and then washed. The stain colors the bacterial cell wall blue and the bacteria would appear blue in the microscope if examined at this point in the procedure. Next the slide is flooded with iodine solution for one minute and then washed again. During
