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the negative stain is an example of an indirect stain in this case the stain seen is applied to the bacteria seen is a negatively charged stain therefore the stain cannot penetrate the liked charge of the cell walls instead the dye colors the background of the cell rendering the cells unstained this method of staining allows for living cells to be observed the negative stain has done in other stains obtain a glass microscope slide and pass it through a flame to sterilize at one end of the slide aseptic we transfer a small drop of 10% seen with a sterile wire inoculating loop transfer a small amount of bacteria to the microscope slide and mix with the drop of singh using a second sterile microscope slide at a 45 degree angle gently spread the drop containing the bacteria across the slide creating a thin film as shown here allow the smear to air dry and then examine under oil immersion if unfamiliar with the proper use and function of a microscope review the video how to use a microscope
