Negate stain in FTM

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Aug 6th, 2022
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How to negate stain in FTM

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hi everyone right now weamp;#39;re going to go over a few techniques that youamp;#39;ll be commonly using in lab these techniques especially the gram stain and the heat fixation will be very important when you guys get to your unknowns so with that letamp;#39;s just jump right into it first as you can see weamp;#39;re going to go over heat fixation and what heat fixation does is it makes the bacteria stick to the slide better and the way that we would do that is we would first add the bacteria to the slide so either if itamp;#39;s um in a test tube weamp;#39;ll just take it right out of the broth and you know take anywhere from one to three loop fulls and let that dry typically the um if you know that your bacteria is gram-negative youamp;#39;ll use more loop fulls just because that gram-negative cells are much smaller than gram-positive cells all right after that weamp;#39;ll let them air dry and make sure that when you do this that theyamp;#39;re comp before you proceed to t

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Some suitable negative stains include ammonium molybdate, uranyl acetate, uranyl formate, phosphotungstic acid, osmium tetroxide, osmium ferricyanide and auroglucothionate. These have been chosen because they scatter electrons strongly and also adsorb to biological matter well.
In contrast to direct stains that bind to bacteria directly, a negative stain colors the background of a smear rather than the bacteria. These stains have negatively charged functional groups so they cant bind directly to negatively charged bacteria.
Some stains commonly used for simple staining include crystal violet, safranin, and methylene blue.
Types of Staining Based on the process, staining is grouped into three types: Simple staining, Differential staining, and Special staining. Differential staining involves multiple stains to distinguish between different types of microorganisms or structures within a specimen.
The main purpose of Negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. eg: Spirilla. It can also be used to stain cells that are too delicate to be heat-fixed.
Special Stains Massons Trichrome. The trichrome stain helps to highlight the supporting collagenous stroma in sections from a variety of organs. Verhoffs Elastic Stain. Reticulin Stain. Giemsa Stain.
Negative Staining Place a drop of the diluted material on a formvar and carbon covered grid. After 30-60 seconds, blot off the excess liquid with filter paper. Add a drop of PTA solution. After 1 minute, blot off the excess with filter paper. Allow the grid to air dry. Examine in the transmission electron microscope.
Commonly used negative stains include uranyl acetate (UA), uranyl formate, ammonium molybdate, and phosphotungstic acid. The technique was invented by Brenner and Horne (1959) and first applied on mosaic viruses mixed with phosphotungstic acid.

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