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hey everybody dr. up in this video weamp;#39;re going to talk about the Gram stain which to me is by far the most important differential or diagnostic stain that we use in microbiology it was developed 1884 by a Danish bacteriologist his name was Hans Christian Graham as the name implies so the reason this is so important is because it tells us for most bacteria it tells us what we need to know about their cell walls it tells us oh grandpa will do several videos about the cell walls but gram-positive cells have really big thick peptidoglycan cell walls gram-negative cells have a really thin cell wall but outside of it they have a lipid outer membrane that has a toxin in it called lipid a and itamp;#39;s very very important to know if youamp;#39;re dealing with a gram positive or gram-negative bacteria because it helps us figure out how to kill them if we need to like for example penicillin and the cephalosporins theyamp;#39;re gonna be useful against the gram positives but not so m