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in this video weamp;#39;re going to look at the negative stain technique now the reason we use a negative stain technique is because it gives us a slide where the bacterial cell is very clear and distinct now youamp;#39;ll recall when you do a bacterial smear you have to heat fix the slide when any time you use heat it distorts the cells with the negative stain Technique we donamp;#39;t use heat so the cells come out more natural you get a much better image now the reason we call it the negative stain technique is because weamp;#39;re applying a stain that carries a negative charge now youamp;#39;ll recall that bacterial cells have a negative charge too so the cells actually repel the stain so weamp;#39;re really staining the background so what you get is a white cell against a dark background you get really nice contrast so letamp;#39;s look at the materials youamp;#39;re going to need to do this technique youamp;#39;ll need a bottle of negatively charged stain Iamp;
