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basic microbiology a laboratory manual negative stain simple negative stain introduction stains simple stains and differential stains simple stains include positive stains and negative stains differential stains staining structures and grouping bacteria such as Gram stain acid-fast stain spores stain capsule stain sill wall stain and nuclear stain positive stains include crystal violet methylene blue stuff running carbon Foxen and malachite green negative stains include never seen convert and in the end positively charged basic stains are attracted to negatively charged bacteria negatively charged acidic stains repelled with negatively charged bacteria purpose to study morphology number and arrangement of bacteria which are difficult to stain such as parakeets or sensitive to heat fixation direct presence of transparent unsustainable capsules such as Cryptococcus neoformans can be used to detect presence of spores prepare samples for electron microscopy purpose single stain advantages
