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[Music] mojo sort is bio legends magnetic separation system the following video will illustrate a typical negative selection protocol using bio legends reagents and magnet [Music] verify you have all the reagents and equipment needed before starting the experiment [Music] prepare a single cell suspension from lymphoid tissues or compatible sample add 10 microliters of biotin antibody cocktail mix well and incubate on ice for 15 minutes resuspend the beads by touching them five times over a vortex set at maximum speed add 10 microliters of nano beads mix well and incubate on ice for 15 minutes add 2.5 milliliters of mojo sort buffer wash the walls of the tube while adding the buffer place the tube in the magnet for five minutes if using a 14 milliliter magnet incubate for 10 minutes do not twist or play with the tube while it is in the magnet pour out the liquid with a clean motion do not shake the tube repeat magnetic separation with labeled cells once more for a total of 2 separation
