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hi friends welcome to biology exams foray.com in the last video we discussed about how to calculate the dilution factor in serial dilution in this video we are going to calculate the number of colony forming units per ml of original stock using a symbol equation the equation is c fu per ml is equal to number of colonies into total dilution factor or tdf divided by volume of culture plated in ml we have divided these into three steps for easy understanding what is the number of cfu per ml of original stock so we need to find out the cfu per ml of this stock solution so this is the question the colony count is given step 1 find out the plate with countable number of colonies so what do you mean by countable number of colonies as a standard procedure select a plate with number of colonies ranging between 30 to 300. so this number is statistically and experimentally deduced number so let me make it more clear let us zoom in this plates suppose this is a blade with 443 number of colonies he